Preparation of an edible product from dough

ABSTRACT

The present invention is directed to mold control and extended shelf life methods and compositions for preparing edible dough-based products, such as, for example, breads, by treating the surface of the dough used to prepare the edible product with at least one preservative and at least one pH adjusting agent prior to or during baking. The present invention is also directed to methods and compositions for preparing edible dough-based products, such as, for example, breads, by treating the surface of the dough used to prepare the edible product with a at least one pH adjusting agent prior to or during baking. The present invention is further directed to improved pan oil compositions for preparing edible dough based products which comprise at least one preservative in an amount effective to inhibit mold growth on the surface of the bread prepared from the dough and at least one pH adjusting agent in an amount effective to improve the activity of the at least one preservative and/or inhibit microbial growth on the surface of the bread prepared from the dough.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of application Ser. No. 10/941,436filed on Sep. 15, 2004 (pending), which claims priority or the benefitunder 35 U.S.C. 119 of U.S. provisional application Nos. 60/504,032,filed Sep. 19, 2003, and 60/565,459, filed Apr. 26, 2004, the contentsof which are fully incorporated herein by reference.

FIELD OF THE INVENTION

The present invention relates to processes and compositions forpreparing edible products from dough and to processes and compositionsfor preparing edible dough-based products having improved moldresistance and extended shelf-life. The present invention also relatesto compositions and baking pan release compositions containingpreservatives.

BACKGROUND OF THE INVENTION

The growth of mold, rope, spoilage yeasts and bacteria is a significantproblem in edible dough-based products, such as, for example, bread andother baked goods. Such microbial growth significantly reduces thecommercial shelf life of the product, increasing the sellers' directcosts due to moldy products that cannot be sold to the consumer andlimiting the time available for storage, distribution, display, sale andconsumption of the product.

Various preservatives are on the market and are used to inhibitmicrobial growth so as to extend the shelf-life of edible dough-basedproducts. See, e.g., E. J. Pyler, Baking Science & Technology, Vol. I,p. 227-236 (3^(rd) Ed. 1988). Examples of such preservatives are sodiumbenzoate, calcium benzoate, potassium benzoate, sodium diacetate,paraben, niacin, calcium acetate, calcium diacetate, sorbic acid, sodiumsorbate, calcium sorbate, potassium sorbate, sodium propionate, calciumpropionate and potassium propionate. However, when used atconcentrations that effectively increase shelf life, preservatives canimpart an off-flavor, odor, color and/or texture (e.g., poor crumbstructure) to the final product that is undesirable to the consumer. Inaddition, preservatives can also inhibit yeast cultures which are usedto prepare the dough-based product, resulting in manufacturing problems,such as, proofing problems, and increasing costs due to the need to usegreater amounts of yeast to offset the yeast inhibition.

Because of the problems associated with the use of preservatives indough-based products, as a compromise, it has been necessary in the artto employ relatively low concentrations of preservatives, that is,preservative concentrations which provide some anti-mold effect, but donot create unacceptable processing conditions due to yeast dosagerequirements or unacceptable impairment to the flavor, odor, colorand/or texture of the product. Accordingly, the prior art discloses theuse of preservatives, such as, calcium propionate, sodium propionate,sorbic acid, potassium sorbate and sodium benzoate, in dough and bakedproducts at very low concentrations. For example, U.S. Pat. No.3,900,570 discloses a maximum usage of calcium propionate of 0.25 partsby weight per 100 parts of flour in the finished dough, with thepreferred range being about 0.06 to about 0.12 parts. U.S. Pat. No.4,416,904 discloses concentrations of 0.04% to 0.10% for sodiumbenzoate, 0.05% to 0.20% for sorbic acid, and 0.4% for calciumpropionate. Similarly, E. J. Pyler, Baking Science & Technology, Vol. I,p. 227-236 (3^(rd) Ed. 1988), discloses that calcium propionate isnormally used in the amount of 2.5 to 3.5 oz/100 lb flour. Morerecently, WO 99/08553 discloses that preservatives, such as, sodium andcalcium propionate, are typically added to bakery products in smallconcentrations in the range of 0.1 to 0.625%, calculated on the weightof the flour.

A number of solutions have been proposed to avoid the problemsassociated with the use of preservatives in edible dough-based products.U.S. Pat. No. 2,997,394, for example, discloses incorporating apreservative into an edible fat having a high melting point, which isthen dispersed throughout the dough. By incorporating the preservativeinto the edible fat composition, it was reported to be possible to usethe preservative in the dough in an amount of about 0.025 to 0.2%, therange depending on the particular preservative, while avoidingunacceptable side effects, such as, yeast inhibition.

Similarly, WO 99/08553 discloses encapsulating a preservative, such as,calcium propionate, into a degradable and edible fatty acid substancewhich is then dispersed in the dough system, and ultimately released.Again, the encapsulation of the preservative is stated to have thebenefit of avoiding inhibition of the microbial culture used to preparethe food product. However, yeast inhibition remains a problem withpreservative encapsulation technologies, presumably caused by leakage ofthe preservative from the encapsulation layer into the dough.

U.S. Pat. No. 6,132,786 proposes another solution for obtaining improvedmold inhibition without impacting the organoleptic properties of thebaked product by using food grade metabolites produced byPropionibacterium sp, instead of traditional preservatives, such as,propionic acid. The metabolites are reported to have a neutral taste,which does not change the flavor of the product, as compared topropionic acid, which is stated to have a distinct unpleasant taste. Themetabolites are also stated to not result in deleterious changes in theconsistency or structural integrity of the finished or stored bakedproduct. Nevertheless, it has been difficult to obtain effective anduniform mold inhibition using propionibacteria metabolites.

In addition to the incorporation of small concentrations ofpreservatives into the dough, preservatives have also been applied tothe surface of the baked product, that is, to the exterior of the bakedproduct after baking. As with the case of the use of preservativesinside the dough, preservatives have been applied to the surface of thebaked product in small amounts.

Hickey, C. S. Bakers Digest, 54 (4), 20 (1980), for example, reportedthat a spray application of a 1.0 to 1.5% sorbate solution on hot,freshly baked breads, buns and rolls, and English muffins was effectiveto increase the shelf-life of the products. The surface treatment wasreported to result in sorbate residuals equivalent to 0.02% based onflour.

Malkki and Rauha, Bakers Digest 52 (1), 47 (1978), disclose an aerosolmethod for mold inhibition in which a solution of propionic acid isatomized at a concentration of 15 mg/liter into an aerosol within anenclosed cooling tunnel through which the bread is conveyed. It isreported that the bread will absorb a concentration of 0.05% of theinhibitor in a 2 mm-thick crust.

He and Hoseney, Cereal Chem, 67 (6), 603-606 (1990), disclose sprayingof calcium propionate solution on the surface of bread after baking andafter cooling the bread for 50 minutes or 10 minutes. The calciumpropionate was added in a very small amount (a 0.2% solution).

Although the addition of preservatives to the surface of the productsafter baking has been proposed as an alternative to adding thepreservative into the dough, because the surface of freshly bakedproducts is fragile and easily damaged, the application of preservativesto the surface of freshly baked products is often undesirable because itcan result in the formation of stripes, discoloration, and/or breakageof the product. In addition baked products, such as, bread, are alsobulky and hard to handle, thus, making it difficult to uniformly andeconomically apply the preservative to the surface of the baked productafter preparation.

Preservatives have also been applied to the dough prior to heating. U.S.Pat. No. 3,021,219, for example, discloses the addition of 0.5% to 10%of the preservative sorbic acid to pan grease to prevent mold growth.Melnick et al., Sorbic Acid as a Fungistat in Bakery Production WithSpecial Emphasis on a Novel Fungistatic Shortening, The Bakers' Digest46 (1956), discloses the use of a fungistatic shortening containingsorbic acid or propionate, which is applied to the surface of the dough.DaSa, Sorbic Acid: Its Use in Yeast-Raised Baker Products, The Bakers'Digest 50 (1966), discloses dispersing the combination of sorbic acidand calcium propionate in a vegetable oil, which is applied to brown'nserve rolls before baking. The treatment was also combined with theaddition of calcium propionate into the dough and by spraying thesurface of the rolls after baking.

In addition to the use in preserving food products, non-toxic, foodgrade preservatives have also been used in pan release compositions invery low amounts for the purpose of preserving the pan releasecomposition. For example, WO 94/22313 discloses the use sorbic acid,acetic acid, phosphoric acid, benzoic acid and propionic acid, in verysmall amounts as preservatives of the pan release compositions. Inparticular, WO 94/22313 discloses a pan release composition comprising0.25% sorbic acid and 0.25% acetic acid, with the percentage of thepreservative being based on the percentage of the pan releasecomposition.

In addition to microbial growth, another major factor which impacts thecommercial shelf life of the edible dough based products is the softnessof the product, which deteriorates during storage in a process commonlyreferred to as staling. The staling of a dough based product, such asbread, is generally characterized by an increase of the firmness of thecrumb, a decrease of the elasticity of the crumb, and changes in thecrust, which becomes tough and leathery. Chemical and enzymatic agentshave both been used in the industry to retard staling. WO 91/04669, forexample, describes the use of a maltogenic alpha-amylase to retard thestaling of baked products.

SUMMARY OF THE INVENTION

The present invention is directed to methods and compositions forpreparing edible dough-based products, such as, for example, breads,buns, rolls, English muffins, cake muffins, bagels doughnuts, tortillas,cakes, biscuits, cookies, pie crusts and pizza crusts, preferably, yeastraised dough products, such as breads.

One aspect of the present invention relates to methods and compositionsfor preparing edible dough-based products by applying a preservativecomposition, preferably, a mold inhibitor, to the surface of dough priorto or during heating. The application of an effective amount of one morepreservatives to the surface the dough prior to or during heating can beused to provide effective microbial inhibition during storage of thedough based product, such as, effective mold inhibition. Although notlimited to any one theory of operation, the methods and compositions ofthe present invention are believed to significantly inhibit spoilagemicrobial growth on the surface of the dough based product, inparticular, during the critical period after heating the dough (e.g.,following baking) and prior to packaging, which results in a significantextension in the shelf life of the dough based product. The methods andcompositions of the present invention are particularly suited forinhibiting spoilage microbial growth on the surfaces of dough basedproducts which are in contact with baking pans during preparation of theproduct, e.g., the bottom and side of a bread which are in contact withthe baking pan, as these are the areas of the dough based product whichare believed to be more susceptible to spoilage microbial growth.

In a preferred embodiment, a pH adjusting agent, such as triacetin,preferably, a pH lowering agent, more preferably, a heat activated pHadjusting agent, is applied to the dough in combination with thepreservative. A particularly preferred pH adjusting agent is triacetin,which, when used in combination with a preservative, as describedherein, can be used to significantly extend the shelf-life of thedough-based product. The pH adjusting agent may be appliedsimultaneously with the preservative, such as, e.g., as part of the samecomposition or through a separate process stream that is appliedsimultaneously, e.g., through the same spray nozzle. Alternatively, thepH adjusting agent may be applied separately (sequentially) from thepreservative on the exterior surface of the dough or dough basedproduct, such as, prior to application of the preservative or afterapplication of the preservative.

When a preservative is applied to the surface of the dough or doughbased product, the preservative can increase the pH in the localizedenvironment of the surface of dough or dough based product. The increasein pH can reduce the effectiveness of the preservative and/or provideconditions that are more optimal for microbial growth. Although notlimited to any one theory of operation, the pH adjusting agent, such astriacetin, is believed to act synergistically with the preservative tofurther preserve and extend the shelf life of the dough based productsby directly inhibiting microbial growth, e.g., by creating pH conditionswhich are not suitable for the microbial growth, and by creating a moreoptimum pH environment for the preservative. In accordance with thepresent invention, a pH adjusting agent is preferably used to lower thepH on the surface of the dough, more preferably, to a pH that at leastcompensates for the pH increase caused by the use of the preservative,and even more preferably, to further lower the pH to more optimal pHconditions for the preservative or for inhibiting spoilage microbialgrowth. By adjusting the pH in the localized environment of the surfaceof the dough, the present invention may also prevent the loss ofpreservative activity at the surface of the product resulting from themigration of the preservative from the surface to the interior.

Yet another aspect of the present invention relates to methods andcompositions for preparing edible dough-based products by applying a pHadjusting agent, such as triacetin, to the surface of dough prior to orduring heating. The pH adjusting agent may be applied to the surface ofthe dough or dough-based product in combination with the application ofa preservative to the surface of the dough or dough-based product, asdescribed herein, or the pH adjusting agent may be applied withoutapplication (simultaneous or sequential) of a preservative on to thesurface of the dough. The pH adjusting agent can itself provide apreservation effect by directly inhibiting microbial growth, e.g., bycreating pH conditions which are not suitable for the microbial growth.The treatment of the surface of the dough or dough-based product with apH adjusting agent may be used in combination with other preservationtechniques, such as, the addition of a preservative into the doughbefore baking.

The preservative and/or a pH adjusting agent, such as triacetin, may beapplied to the surface of the dough or dough based product in anysuitable manner, such as, by treating or coating the dough, including,by treating or coating a surface which contacts or holds the doughduring processing of the dough and preparation of the edible product,such as, e.g., a baking pan, a container, packaging, a conveyor, or acutting knife. In a preferred embodiment, the preservative compositionand/or pH adjusting agent is applied to the surface of the dough bytreating a pan used to prepare the dough based product, preferably,prior to filling the pan with the dough. In preferred embodiment, thepreservative is applied to the surface of the dough in amount to obtainat least 0.05% of the preservative in the surface of the productprepared from the dough (e.g., crust of the bread), more preferably,0.05 to 5%, preferably, 0.05 to 2%, such as, 0.05 to 0.5% of thepreservative in the surface of the product or a surface which is incontact with the product (e.g., a pan, container, etc.) prepared formthe dough.

The preservative composition and/or pH adjusting agent may also beapplied to the dough by treating, e.g., spraying, the surface of thedough, prior to or after adding the dough to a pan, and/or by treatingthe surface of the dough or dough based product which is not in contactwith the surface of the cooking pan, such as, by treating (e.g.,spraying) the top or other exposed surface of the dough.

The treatment of the surface of the dough with a preservativecomposition and/or pH adjusting agent (prior to or during cooking) mayalso be used in combination with the addition of a preservative onto thesurface of the edible product after cooking (e.g., after baking).

The treatment of the surface of the dough as described herein, may alsobe used in combination with the addition of an amount of preservative inthe dough, that is, inside the dough rather than on the surface of thedough. Accordingly, in a preferred embodiment, the present inventionrelates to methods of producing dough based products by applying atleast one preservative in the dough and by applying at least onepreservative and/or pH adjusting agent on the surface of the dough,wherein the combination of the preservative in the dough and on theapplication of the preservative and/or pH adjusting agent on the surfaceof the dough provides the desired microbial inhibition during storage.

Although the addition of a preservative and/or pH adjusting agent on thesurface of the dough or dough based product may be used in combinationwith the addition of an amount of preservative inside the dough or thedough based product, the present invention is preferably used to reduceor even eliminate the use of preservatives added in the dough, and thusto reduce or avoid the problem of yeast inhibition (and consequent needto increase yeast dosages to offset the inhibition during processing) aswell as the problems in off-flavor, odor, color and/or texture resultingfrom the use of preservatives in dough. More preferably, the ability toreduce or eliminate the use of preservatives added in the dough isachieved by the application of the preservative to the surface of thedough in combination with the treatment of the surface of the dough ordough based product with a pH adjusting, preferably, a pH loweringagent, which significantly enhances the activity of the preservative,more preferably, a pH adjusting agent which is activated at bakingtemperature. Such higher surface concentrations of preservative can beobtained by the surface application of the preservative as describedherein.

The present invention further relates to methods and compositions forpreparing dough based products and dough based products having a highconcentration of a preservative in the surface of the dough basedproduct (e.g., bread crust), such as, at least 0.05% of the preservativein the surface of the dough based product (e.g., crust of a bread), morepreferably, 0.05 to 5% of the preservative in the surface of the doughbased product, and having a low concentration, including 0%, of apreservative in the dough based product (e.g., in the bread crumb), suchas, less than 0.5%, less than 0.1%, less than 0.01%, less than 0.001% ofthe preservative in the dough based product, more preferably between 0to 0.5% of the preservative in the dough based product.

Any suitable composition may be used to apply the preservative and/or pHadjusting agent to the dough or dough based product, including aqueouscompositions, oil based compositions, and emulsions. In a preferredembodiment, the composition is an oil based composition, such as, forexample, an oil composition having an oil as the main carrier or theonly carrier component, or a composition comprising an oil as one of themain components of the composition, such as, a water-in-oil emulsion, anoil-in-water emulsion or a composition comprising a mixture of oils,waxes and lecithin. In a more preferred embodiment, the composition is asprayable composition, more preferably, a composition that can beapplied to the dough or to a surface which contacts the dough duringprocessing of the dough into the bread, e.g., a pan, in a pre-ovenspraying process.

Any suitable preservative may be used, including oil solublepreservatives, such as, e.g., glycerol tripropionate, or water solublepreservatives, such as, e.g., calcium propionate or sorbic acid. Morepreferably, and in particular, when using preservatives that are notsoluble in oil compositions, such as, in sprayable oil compositions, thepreservative and/or pH adjusting agent should preferably be in the formof finely dispersed particles. Although not limited to any theory ofoperation, the use of fine preservative particles, that is, having asmall maximum particle size, and fine pH adjusting agents providesproper dispersion or suspension of these components in the composition,and provides superior preservation when applied to the dough.

When the composition is an oil composition and the preservatives and/orthe pH adjusting agents are not oil soluble, the composition alsopreferably comprises a high melting fatty substance (particles) and/orwax substance (particles). Preferably, the fatty substance and/or waxsubstance has a melting point above 40° C., more preferably above 50°C., above 60° C., or above 65° C. Although not limited to any one theoryof operation, the fatty substance and/or wax particles are believed tofurther maintain proper dispersion or suspension of the preservativeparticles and/or pH adjusting agents in the oil based composition and topromote the effective application of the components to the dough and/ordough based product. However, if the preservative and/or pH adjustingagents are extremely fine, the use of fatty material and/or wax materialto obtain the desired dispersion may preferably be reduced or avoided.

The preservative and/or pH adjusting agent are preferably applied incombination with a pan release agent. Preferably, such components areapplied simultaneously, such as, as components of a pan releasecomposition, that is, a pan release composition comprising (i) at leastone pan release agent and (ii) at least one preservative and/or at leastone pH adjusting agent in an amount effective to inhibit mold growth inthe dough based product prepared in a pan treated with the pan releasecomposition. More preferably, the pan release composition comprises botha preservative and pH adjusting agent. Accordingly, another aspect ofthe present invention is directed to pan release compositions comprisingone or more preservatives and/or one or more pH adjusting agents in anamount effective to inhibit spoilage microbial growth during storage ofthe dough-based product prepared in a pan treated with the pan releasecomposition, and to pans treated or coated with such compositions.

The preservative and/or pH adjusting agent may also be applied to adough simultaneously by application to a cooking pan (e.g., baking panor frying pan) used to prepare a dough based product by a separateprocess stream or composition applied at the same time as the panrelease composition, e.g., through an application system having twoseparate process streams that are applied simultaneously to the pan,such as, by spraying simultaneously through the same spray head.

Alternatively, the preservative and/or pH adjusting agent are appliedsequentially in combination with a pan release composition in thedesired order, e.g., the pan release composition may be applied first,followed by the preservative and/or a pH adjusting agent. Alternatively,the preservative and/or pH adjusting agent may be applied first,followed by the pan release composition. In addition, combinations ofsimultaneous and sequential applications may also be used, as desired,for example, the pan release composition may be applied first followedby the simultaneous application of both the preservative and the pHadjusting agent, or simultaneous application of the preservative and panrelease agent, followed by application of a pH adjusting agent.

Yet another aspect of the present invention relates to methods andcomposition for treating prepared dough based products, that is, afterheating the dough to produce the product, by treating the prepared doughbased product with a preservative and/or pH adjusting agent, preferably,shortly after heating, such as, immediately following (i.e., seconds tominutes after the product leaves the oven or cooking process, e.g., 1 to30 minutes, preferably 1 to 15 minutes). Such processes may preferablybe used in combination with the treatment of the dough prior to orduring heating, as described herein, and in combination with otherpreservation methods, such as, the addition of a preservative inside thedough.

Yet another aspect of the present invention is directed to thepreservation methods and compositions described herein in combinationwith anti-staling methods and compositions, preferably, one or moreanti-staling enzymes, more preferably, a maltogenic alpha-amylase, andeven more preferably, a maltogenic alpha-amylase which is added into thedough prior to heating the dough. In a preferred embodiment of thisaspect of the invention, the present invention provides a process forpreparing an edible product comprising applying one or morepreservatives and/or one or more pH adjusting agents to the surface ofthe dough, and heating the dough, wherein the dough preferably comprisesone or more anti-staling agents, more preferably, one or moreanti-staling enzymes, and even more preferably, a maltogenicalpha-amylase.

Yet another aspect of the present invention is directed to methods forpreparing dough based products for inventory, such as, for example,methods of baking bread for inventory. In particular, the presentinvention also provides methods for preparing dough-based products whichcan be stored for a longer period of time following preparation, andbefore delivery or sale, and/or which can be distributed over greaterdistribution network, over a greater distance from the production siteand/or by a slower distribution method.

Some of the factors restricting the ability to significantly extendshelf-life of dough-based products have been the problems of yeastinhibition, off-flavor, odor, taste and/or texture resulting fromconcentrations of preservatives which are necessary to obtain the longershelf-life. Accordingly, the present invention provides methods andcompositions for applying an effective amount of a preservative to thedough-based product so as to obtain a significant extension in shelflife, but to still produce a commercially acceptable product, that is, aproduct having suitable organoleptic and structural properties, and byan economical production process, that is, by avoiding excessive yeastdosage requirements. Thus, when the spoilage microbial inhibitionmethods of the present invention are used in combination withanti-staling agents, preferably, anti-staling enzymes, more preferably,an anti-staling effective amount of a maltogenic alpha-amylase, asignificant extension in shelf life can be obtained, as defined by bothmicrobial inhibition during storage (e.g., mold inhibition) and thesoftness of the product (anti-staling). In preferred embodiments, thepresent invention is directed to dough based products that arecharacterized by having a longer shelf life than dough based productsprepared by other methods, e.g., a shelf life of at least 20 days to 90days, as defined by mold inhibition and softness of the product.

Yet another aspect of the present invention relates to the applicationof very high amounts of preservatives to dough based products, inparticular, 2.0 to 20% preservative solution, such as, 10 to 20%preservative solution, may be applied to the surface of the dough and/orto the surface of a baked product without resulting in unacceptableyeast inhibition, off-flavor, odor, taste and/or texture problems.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to methods and compositions forpreparing edible dough-based products by applying a preservative,preferably, a mold inhibitor, and/or pH adjusting agent to the surfaceof a dough or dough based product. Advantageously, the methods andcomposition described herein can be used to significantly extend theshelf life of edible dough based products, such as, bread, tortillas,cakes, pancakes, biscuits, cookies, pie crusts, more preferably bakedproducts, such as, bread products.

The dough used to prepare the dough based product generally comprisesflour, e.g., from grains, such as, wheat flour, corn flour, rye flour,oat flour, or sorghum flour. The dough is generally leavened by theaddition of a suitable yeast culture, such as a culture of Saccharomycescerevisiae (baker's yeast) or a chemical leavening agent.

The edible dough based product may preferably be any kind of bakedproduct prepared from dough, either of a soft or a crisp character,either of a white, light or dark type. Preferred edible dough basedproducts include bread (in particular white, wheat, whole-meal,low-carb, brown, multi-grain, dark and rye bread), typically in the formof loaves, buns or rolls, and more preferably, pan bread, hamburgerbuns, French baguette-type bread, pita bread, tortillas, cakes,pancakes, biscuits, cookies, pie crusts, crisp bread, steamed bread,pizza crust and the like.

The edible dough based product may also be prepared by frying (e.g. deepfrying in hot fat or oil). An example of such an edible product is adoughnut.

As used herein, a “preservative” is an agent which inhibits the growthof mold, rope, spoilage yeasts and/or bacteria on or in the edible doughbased product during storage. Any suitable preservative may be used,including combinations of preservatives. Preferably, the preservative isan anti-mold agent, also referred to herein as a “mold inhibitor.”Non-limiting examples of preservatives include sodium benzoate, calciumbenzoate, potassium benzoate, sodium diacetate, paraben, niacin, calciumacetate, calcium diacetate, sodium sorbate, calcium sorbate, potassiumsorbate, sodium propionate, calcium propionate, potassium propionate,sorbic acid, and acetic acid. Other preservative include a bacteriocin,nisin and natamycin. In preferred embodiments, combinations ofpreservatives are used, including combinations of the previous list ofpreservatives, to provide a synergistic effect.

In preferred embodiments, the preservative is water-soluble, such as,propionic acid or a propionate, e.g., calcium propionate, a sorbate,e.g., potassium sorbate, a benzoate, e.g., sodium benzoate, or citricacid. In other preferred embodiments, the preservative is oil-soluble,such as, e.g., a propionic acid ester such as glycerol mono-, di- ortri-propionate (mono-, di- or tripropionin). Sorbic acid, propionic acidor a volatile ester such as ethyl propionate may also be used. In otherpreferred embodiments, the preservative may be dissolvable in alcohol(ethanol), such as, e.g., sorbic acid.

The selection of the preservative preferably depends on the pH of thedough or dough based product, which is an important factor for both theundesired inhibitory effect of the preservative on the yeast duringpreparation of the product and the desired microbial inhibition (e.g.,mold inhibition) which occurs during storage of the product.Accordingly, the pH conditions preferably should be optimized accordingto the preservative used. For example, propionate is generally mold andyeast inhibitory when it is in the acid form (propionic acid), and theequilibrium between propionate and propionic acid is dependent on pH.Propionate, for example, is therefore not very efficient in breadprocedures where the pH of the crumb or surface of the bread is high(above 5.5) and far more efficient in bread produced by the sponge anddough procedure where the pH of the bread crumb or surface of the breadis lower (around 5.0), and it is even more efficient in bread producedby sour dough techniques. Other preservatives have different pHoptimums, as are known in the art. Accordingly, the pH should alsopreferably be considered when the preservative is selected and/or theamount of preservative is optimized.

The preservative is applied in an amount effective to inhibit mold,rope, spoilage yeast, and/or bacteria growth during storage of thedough-based product. Microbial inhibition (during storage) is usuallydetermined by visual inspection for microbial growth, such as, when thefirst mold growth appears on the product to the unaided eye. The amountof preservative added will vary depending on the shelf-life desired, aswell as the type of edible product being prepared, that is, a greaterconcentration of preservative should be added if a longer shelf life isdesired. Optimization of the amount of preservative can be performedusing dosage optimization experiments. In preferred embodiments, thepreservative is applied in an amount such that the first spoilagemicrobial growth (e.g., mold) does not appear on the product until atleast 5 days from cooking (e.g., baking), more preferably, at least 6days, at least 7 days, at least 8 days, at least 9 days, at least 10days, at least 11 days, at least 12 days, at least 13 days, at least 14days, at least 15 days, at least 16 days, at least 17 days, at least 18days, at least 19 days, at least 20 days, at least 21 days, at least 22days, at least 23 days, at least 24 days, at least 25 days, at least 26days, at least 27 days, at least 28 days, at least 29 days, at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days, at least 90 days, from preparation (e.g.,baking), as can determined by visual inspection after the product isstored at ambient temperatures and humidity in closed polyethylene bagsafter the bread has cooled down after baking unwrapped for about 10minutes to about 3 hours.

Further embodiments of the present invention are directed to methods andcompositions for inhibiting microbial growth (preferably, mold growth)in dough-based products by applying a preservative to the surface ofdough in an amount of at least 0.01 to 5 milligrams of the preservativeper cm² of the surface of the dough prior to or during cooking (baking),preferably 0.1 to 2 milligrams of the preservative per cm² of thesurface of the dough prior to or during cooking.

The amount of preservative applied will vary depending on the locationin which the product is being prepared, distributed, or sold or duringthe period for consumption. For example, as is known in the art,preservative usage varies depending on the region, including, theclimate and conditions of the manufacturing facility, and depending onthe temperature, humidity, and rain amount of the region. As shown, forexample, in the following table (which presents examples typical dosagesof the preservative calcium propionate added into the dough to obtaintraditional 12 to 18 day shelf life by region), the calcium propionatelevel added into the dough will vary depending on the region:

REGION (United States) % of Calcium Propionate Pacific Northwest  0-0.18 California 0.2-0.3 Upper Central 0.15-0.25 Lower Central0.2-0.3 East Coast/Northern 0.2-0.3 Southeast Coast 0.3-1.0

Similarly, such environmental factors should therefore also be considerwhen applying the preservative to the surface of the dough, andpreferably, the amount of preservative added is optimized based on thetype of product, the region of manufacture, the facility of manufacture,and the method of manufacture.

The amount of preservative added to the surface of the dough will alsovary depending on whether a preservative, preferably, a small amount, isapplied into the dough, such that the desired microbial inhibition(e.g., mold inhibition) in or on the product is based a combination ofthe preservative on the surface of the dough. However, as show inExample 4, it is believed that only very little amount of thepreservative will be present in the surface of the product (e.g., crustof bread) based on the addition of the preservative into the dough. Insome embodiments, it is preferred to add a preservative into the dough,preferably, at a low dosage, such as, less than 0.5% by weight relativeto the flour in the dough, more preferably, between 0.05-0.5%, e.g.,0.1-0.2%, in combination with the addition of the preservative to thesurface of the dough, as described herein.

The amount of preservative added to the surface of the dough will alsovary depending on whether a preservative, preferably, a small amount, isapplied onto the surface of the dough-based product after cooking, forexample, after baking. Thus, the amount added to the surface of thedough should also be adjusted based on the amount of preservative thatwill subsequently be added to the dough based product after cooking.

Controlling pH, in particular, the pH of the surface of the dough ordough based product, can be used to significantly improve spoilagemicrobial inhibition as defined by this invention, preferably by using apH adjusting agent. As used herein, a “pH adjusting agent” is an agentwhich can be used to adjust the pH of surface of the dough or doughbased product. Preferably, the pH adjusting agent is a pH loweringagent, such as, triacetin (102-76-1). More preferably, the pH adjustingagent is a temperature (heat) activated pH adjusting substance which, atbaking temperatures, is broken down into components which will cause areduction in the pH in the localized environment of the surface of thedough or dough-based product. In another preferred embodiment, the pHadjusting agent is an agent which converts from a solid or liquidcomposition to a vapor or gas composition at baking temperatures ofabout 150° C. to 300° C., more preferably about 230° C. for bread(420-460° F.), wherein the dough and or dough-based product is treatedwith the pH adjusting agent in vapor or gaseous form, e.g., when vaporor gaseous pH adjusting agent is circulated in a treating chamber, suchas, an oven, containing the dough or dough-based product.

Examples of suitable pH adjusting agents include, e.g., triacetin,monocalcium phosphate, acetic acid, citric acid, pyrophosphate, sodiumphosphate, potassium phosphate, and combinations thereof. A particularlypreferred pH adjusting agent is triacetin, which when used incombination with a preservative in the surface treatment of dough anddough based products can significantly extend shelf life of dough basedproducts.

The pH adjusting agent is applied in an amount effective to alter the pHof the surface of the dough or dough based product, more preferably tolower the pH of the surface of the dough product, and more preferably,to lower the pH of the surface of the dough based product during theperiod before packaging of the dough based product, such as, duringcooling of the product following cooking.

When used in combination with a preservative, preferably, the pHadjusting substance should be used in an amount effective to counter orat least partially counter any pH increase at the surface caused by thepreservative and/or to improve the activity of the preservative on thesurface of the dough and/or dough-based product (e.g., bread). Morepreferably, the pH adjusting agent should be used in an amount effectiveto obtain more optimal pH conditions for inhibiting spoilage microbialgrowth and to thereby inhibit microbial growth on the surface of thedough and/or dough-based product. In another preferred embodiment, thepH adjusting agent may also be added in an amount effective to lower thepH of the surface of the dough and/or dough-based product by a factor ofat least 0.05 pH units, more preferably, at least 0.01 pH units, morepreferably, at least 0.1 pH units, at least 0.5 pH units, at least 1 pHunit, at least 1.5 pH units, or at least 2 pH units, such as, 0.1 to 2pH units, 0.1 to 1.5 pH units, 0.1 to 1 pH units, and 0.1 to 0.5 pHunits. The pH adjusting agent can also be used to adjust the pH to atarget pH (target pH for the preservative or bread composition), e.g.,the target pH for bread is preferably pH 4.9 to 5.1. Optimization of theamount of pH adjusting agent can be performed using dosage optimizationexperiments.

The preservative and/or pH adjusting agent may be applied to the surfaceof the dough or dough based product in any suitable application process,preferably, by coating the surface of the dough or dough based productwith an effective amount of a preservative and/or an effective amount ofa pH adjusting agent, including by treating or coating a surface whichcontacts or holds the dough or dough based product (e.g., pans,containers, packaging, pans, cutting knives, conveyors, and lids (e.g.,for lidded bread and dough products). In a preferred embodiment of thepresent invention, a preservative and/or a pH adjusting agent, isapplied to the surface of the dough or dough based product by applyingthe preservative to the pan used to prepare the dough based product,(e.g., a baking pan).

In a preferred embodiment, the preservative and/or the pH adjustingagent are applied by spraying the dough or dough based product with thepreservative and/or pH adjusting agent. More preferably, the dough ordough based product is sprayed with an aqueous composition (e.g., aconcentrated solution of the preservative and, preferably, a pHadjusting agent) or oil based composition.

In another preferred embodiment, the preservative and/or the pHadjusting agent are applied by passing the dough or dough based productthrough a vapor containing the preservative and/or pH adjusting agent.More preferably, the dough or dough based product may be passed throughvapors of a volatile preservative (such as, propionic acid or ethylpropionate) and/or a volatile pH adjusting agent. In a preferredembodiment, the preservative and/or pH adjusting agent are applied by anaerosol method in which a solution of the preservative and/or pHadjusting agent is atomized into an aerosol within an enclosed tunnelthrough which the dough or dough based product is conveyed.

In another preferred embodiment, the preservative and/or pH adjustingagents are applied to the surface of the dough by applying thepreservative and/or pH adjusting agent to the pan used to prepare thedough based product, and preferably, the preservative and/or pHadjusting agent are also applied to the portion of the dough which isnot in contact with the pan, for example, by spraying the exposedsurface of the dough.

The methods and compositions of applying preservatives and/or pHadjusting agents to the surface of dough or the dough-based product, asdescribed herein, may also be combined with other preservation methodsto obtain the desired microbial inhibition during storage, including,adding a preservative into the dough, as is known in the art, and addinga preservative onto the surface of the product after cooking (e.g.,baking). In a preferred embodiment, a preservative, e.g., at least 0.05%to 2% based on the weight of flour, more preferably 0.05 to 0.5% basedon the weight of flour, is added into the dough in combination with theaddition of a preservative to the surface of the dough, as describedherein.

In another preferred embodiment, a preservative and/or pH adjustingagent are applied to the surface of the dough before heating (e.g.,before baking), as described herein, and then an additional desiredamount of a preservative and/or pH adjusting agent are applied to thesurface of the product after heating (e.g., after baking). For example,in a preferred embodiment, an additional amount of a preservative and/orpH adjusting agent is added to a post-oven topping system or edible glue(e.g., comprising starch or a gum) which is applied (e.g., sprayed orcoated) on the product after the heating. Examples are seed adhesionsystems applied to hamburger buns after baking, cereal and seed toppingsapplied to bread loaves and top coating or icing applied to doughnutsafter frying. Preferred examples of post topping applications includethe use of edible glues, such as, starch-based glues which contain thepreservative and/or pH adjusting agent.

Preferably, non-chemical microbial inhibition (e.g., mold inhibition)methods and compositions are also used, such as, for example,sterilizing packaging and sterilizing the machinery and plant, such as,for example, by using germicidal ultraviolet rays to sterilize theatmosphere in which the products are exposed. See, e.g., E. J. Pyler,Baking Science & Technology (3^(rd) Ed. 1988).

Any suitable composition may be used to apply the preservative and/or pHadjusting agent to the dough or dough based product, including aqueouscompositions, oil based compositions and emulsions, wherein thepreservative and/or pH adjusting agent is dissolved, suspended,dispersed, or partially dissolved or partially suspended in suchcomposition, as appropriate. As used herein, “an oil based composition”includes an oil composition having an oil as the main carrier or theonly carrier component, or a composition comprising an oil as one of themain components of the composition, such as, a water-in-oil emulsion, anoil-in-water emulsion or a composition comprising a mixture of oils,waxes and lecithin. In a more preferred embodiment, the composition is asprayable composition, more preferably, a composition that can beapplied to the dough in a pre-oven spraying process.

The preservative and/or pH adjusting agent composition is preferably anoil-based composition. Suitable oils include animal, vegetable orsynthetic oil, or an oil fraction selected from the groups consisting ofa food grade mineral oils, coconut oil, palm oil, palmkernel oil, soybean oil, corn oil, cottonseed oil, sunflower oil, ground nut oil,safflower oil, tallow, high erucic rape oil, low erucic rape oil andmixtures thereof. Preferably, the oil is a high temperature stable oil.

The preservative particles and/or pH adjusting agent particles arepreferably finely dispersed, having a maximum particle size below 1000μm, below 900 μm, below 800 μm, below 700 μm, below 600 μm, morepreferably, below 500 μm, below 400 μm, or below 300 μm. Morepreferably, the particles have a maximum particle size below 200 μm,below 150 μm, below 100 μm, below 75 μm, below 50 μm, below 40 μm, orbelow 30 μm. Particle size may be determined using standard process andequipment known in the art, such as, e.g., a laser particle counter, andthe desired maximum particle size may be obtained by any suitablemethod, such as, by sieving the particulate material.

The use of finely dispersed particles is particularly preferred when thepreservative and/or pH adjusting agent composition is an oil basedcomposition, and when the preservative and/or pH adjusting agent are notoil soluble, e.g., an oil based composition comprising calciumpropionate, and more preferably, in such compositions, the preservativeparticles and/or the pH adjusting agent particles should have a particlesize below 200 μm, below 150 μm, below 100 μm, below 75 μm, below 50 μm,below 40 μm, or below 30 μm.

Notwithstanding the above, some preservative particles may be larger,e.g., at most 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 25%,50%, 60%, 70%, 80%, 90% of the particles may be larger than a statedminimum. For example, a suitable calcium propionate may contain at most1% by weight of particles larger than 500 μm. Another suitable calciumpropionate may contain at most 5% by weight of the particles larger than250 μm, or 60% of the weight of the particles larger than 125 μm, or 95%of the weight of the particles larger than 90 μm.

For an oil based preservative and/or pH adjusting composition, thecomposition should preferably also comprise a fatty substance and/orwax, more preferably, a high melting fatty substance or wax in an amountto maintain the dispersion of the particles in the oil-based compositionand to promote the effective release of the preservative and/or pHadjusting agent. More preferably, the preservative and/or pH adjustingcomposition comprises from 0.5% to 20% of high melting fatty substance(particles) and/or wax substance (particles), preferably between 1% to10% of a high melting fatty substance and/or wax.

Suitable fatty substances include, e.g., triglycerides. Suitable waxesinclude, e.g., Carnauba wax, jojoba wax, bees wax, sugar cane wax,bayberry wax, and Candellila wax, preferably having a particle diameterof at least 10 μm meter to 1000 μm meter as measured with a laserparticle counter.

In addition to the carrier for the preservative composition and/or pHadjusting agent composition, such as, for example, an oil-based carriercomprising the preservative particles and/or pH adjusting agent, thecomposition may also include other components useful for enhancing mold,rope, spoilage yeasts and/or bacteria inhibition and/or for adhering thepreservative to the dough, pan or dough-based product, such as, forexample, a starch or other food grade acceptable agent which will aidadherence of the preservative and/or pH adjusting agent to the dough orpan. In other preferred embodiments, a topping and glazing may also beused in combination in the preservative and/or pH adjusting compositionsdescribed herein.

In preferred embodiments, the preservative and/or the pH adjusting agentare applied in combination with a pan release agent. In a preferredembodiment, the preservative and/or the pH adjusting agent are appliedas components of a pan release composition, that is, a pan releasecomposition which includes at least one pan release agent, and examplesof such compositions and agents are well-known in the art. The panrelease composition may preferably be an emulsion (water-in-oil oroil-in-water) containing a water-soluble preservative and/or watersoluble pH adjusting agent together with conventional pan releaseagents/ingredients, such as, vegetable oil, animal fat, refined mineraloil, mono- and diglycerides, polysorbate, polyoxyethylene ester,lecithin and polyglycerol polyricinolate. In another preferredembodiment, the composition is a pan oil (trough grease) comprising apreservative and/or pH adjusting agent which is soluble or dispersiblein oil together with conventional ingredients, such as, vegetable oil(e.g. soybean oil, hydrogenated soybean oil or coconut oil), a wax(e.g., carnauba wax), purified mineral oil (e.g. white mineral oil), andlecithin. The pan release composition may be formulated in analogy withU.S. Pat. No. 4,547,388, U.S. Pat. No. 5,472,482, WO 2002/071864 and WO2002/013623.

Suitable oils for use as the oil component are animal, vegetable orsynthetic oils, or oil fractions selected from the groups consisting ofcoconut oil, palm oil, palmkernel oil, soy bean oil, corn oil,cottonseed oil, sunflower oil, ground nut oil, safflower oil, tallow,high erucic rape oil, low erucic rape oil, rape seed oil and mixturesthereof. Suitable oils also include high oleic oils, includingfractionated high oleic oils.

To obtain a more pasty consistency, the pan release agents of hard fatsmay be included in the pan release composition. Examples of suitablehard fats include at least partially hydrogenated coconut oil, palm oil,palm oil oleine and stearine, palm kernel oil, cottonseed oil, soy beanoil, sunflower oil, ground nut oil, olive oil safflower oil, tallow,lard, butter fat, high erucic acid rape seed oil and inter-esterifiedmixtures thereof.

The preservative and/or the pH adjusting agent, are included in the panrelease composition in an amount effective to inhibit microbial growth,preferably mold growth, during storage of the product, when a doughbased product is prepared in a pan treated with the pan releasecomposition. Preferably, the preservative is incorporated in the panrelease composition in an amount of at least 1%, at least 2%, at least3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, atleast 9%, at least 10%, at least 11%, at least 12%, at least 13%, atleast 14%, at least 15%, at least 20% by weight of the pan releasecomposition, such as, 1-50%, 2-50%, 1-30%, 3-20% or 5-15% by weight ofthe pan release composition. The amount of the preservative in the panrelease composition may preferably correspond to 0.05-0.2% relative tothe flour in the dough. In other preferred embodiments, the preservativeis included in the pan release composition in an amount of at least0.05%, at least 0.06%, at least 0.07%, at least 0.08%, at least 0.09%,at least 0.1%, at least 0.2%, at least 0.3%, at least 0.4%, at least0.5%, at least 0.6%, at least 0.7%, at least 0.8%, at least 0.9%relative to the flour in the dough.

The pH adjusting agent is preferably included in the pan releasecomposition in an amount of at least 1%, such as, at least 2%, at least3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, atleast 9%, at least 10%, at least 15%, at least 20%, at least 25%, atleast 30%, at least 35% or at least 40%, such as in the range of 1 to40%, 1 to 40%, 2 to 40%, 3 to 40%, 4 to 40%, 5 to 40%, 6 to 40%, 7 to40%, 8 to 40%, 9 to 40%, 10 to 40%. In other preferred embodiment, thepH adjusting agent is included in an amount of at least at least 10 to30%, 10 to 20%, 10 to 15% or 10 to 10%, at least 10 to 30%, 10 to 20%,10 to 15%, at least 9 to 30%, 9 to 20%, 9 to 15% or 9 to 10%, at least 8to 30%, 8 to 20%, 8 to 15% or 8 to 10%, at least 7 to 30%, 7 to 20%, 7to 15% or 7 to 10%, 6 to 30%, 6 to 20%, 6 to 15% or 6 to 10%, at least 5to 30%, 5 to 20%, 5 to 15% or 5 to 10%, at least 4 to 30%, 4 to 20%, 4to 15% or 4 to 10%, at least 3 to 30%, 3 to 20%, 3 to 15% or 3 to 10%,at least 2 to 30%, 2 to 20%, 2 to 15% or 2 to 10%, at least 1 to 30%, 1to 20%, 1 to 15% or 1 to 10%.

The pan release composition is then applied to a pan used to cook (e.g.,bake) the dough. The pan release composition may be applied to the panin any suitable manner, such as, e.g., by coating the pan, spraying thesurface of the pan or by dipping the surface of the pan in a solutioncomprising the pan release composition. For a typical bread pan size of8.5 cm high, 12 cm wide and 32 cm long, the weight of the oil-based panrelease agent is preferably between 0.5 to 2 g pan oil per pan. Inpreferred embodiments, the pan release composition is applied in anamount of 0.5 to 2 mg/cm² of the surface of the pan, more preferably,1-2 mg/cm² of the surface of the pan, such as, e.g., 0.78 mg/cm² of thesurface of the pan.

The amount, percentage or effect of the preservative and/or pH adjustingmay be based on a single agent or a combination of agents.

In another preferred embodiment, the preservative and/or pH adjustingagent are applied to the pan in combination with but separately from thepan release composition, such as, for example, by spraying the pan witha pan release composition and separately by spraying the pan with apreservative composition and/or pH adjusting agent, in the desiredorder. In another embodiment, the pan release composition and thepreservative composition and/or the pH adjusting agent can be applied tothe pan simultaneously, for example, by a separate process stream orcomposition that is applied to the pan at the same time as the panrelease composition process stream, e.g., through a spray system havingtwo separate process streams that are sprayed simultaneously through thesame spray head.

The present invention also provides pans pre-treated prior to use with acomposition comprising a preservative and/or pH adjusting agent, morepreferably, a pan release composition comprising these components, asdescribed herein, in an amount effective to inhibit microbial growth(e.g., mold growth) during storage of a dough based product prepared inthe pan treated with these compositions.

The preservative and/or pH adjusting agent composition, including as apan release composition, may optionally include a flavoring agent.Examples of flavoring agents are butter flavor, bread flavor, breadcrust flavor, bread crumb flavor, vanilla flavor, meat flavor, and/orflavor precursors that create similar flavors, such as, precursors whichare activated during the baking process (i.e., heat activated). Theflavoring agents are particularly preferred when higher concentrationsof preservatives are used at the surface. In some preferred embodiments,preservative and/or pH adjusting agent are included as components (e.g.,dissolved or dispersed) in a flavor composition which is applied to thesurface of the dough or dough based product, as described herein.

The edible dough based products prepared by the methods of the presentinvention, in preferred embodiments, have a high concentration of apreservative in the surface of the dough based product, for example, inthe crust of the bread. In more preferred embodiments, the dough basedproducts have at least 0.01 to 1%, such as, at least 0.05% or at least0.08% of the preservative on the surface of the product prepared fromthe dough (e.g., crust), more preferably, at least 0.1 to 2.0% of thepreservative on the surface of the product. In another preferredembodiment, a bread is prepared to have at least 0.025% of apreservative in the crust of the bread one day after the bread is baked,at least 0.05% of a preservative in the crust of the bread one day afterthe bread is baked, or at least 0.08% of a preservative in the crust ofthe bread one day after the bread is baked. In yet another preferredembodiment, a bread is prepared to have at least 0.05% to 0.5% of apreservative in the crust of the bread.

The edible dough based products also preferably have a relatively lowconcentration (e.g., at least 0.05% to 0.5%), including no concentration(0%), of preservative inside the dough based product (e.g., the breadcrumb). In a preferred embodiment, the present invention is directed toa bread having at least 0.05% of the preservative on the surface (e.g.,the bread crust), more preferably at least 0.05% to 0.5% of thepreservative on the surface and having less than 0.5% of a preservativein the product (e.g., the crumb), more preferably less than 0.25% of apreservative in the product, more preferably less than 0.2% of apreservative in the product, and even more preferably less than 0.1% ofa preservative in the product, including 0%. When the preservative isapplied to obtain such desired levels on the surface of the dough basedproduct, the application of the preservative is preferably incombination with a pH adjusting agent, more preferably, triacetin.

The softness of the dough based product, in particular, bread productsand the like, is preferably maintained by the use of one or moreanti-staling agents or softners, such as, emulsifiers, hydrocolloids andenzymatic anti-staling agents. Advantageously, the combined use of ananti-staling agent and a preservative, preferably an anti-mold agent,and/or pH adjusting agent applied according to the invention can retardboth microbial growth (such as, mold growth) and staling (e.g., crumbfirming) of the product, and provide a product suited for long-termstorage, e.g., for over two weeks, for over three weeks, or for overfour weeks. As used herein, an anti-staling agent refers to a chemical,biological or enzymatic agent which can retard staling of thedough-based products, that is, which can reduce the rate deteriorationof the softness of the dough based product during storage. The softnessof dough based products (and the anti-staling effect of the anti-stalingagent) can be evaluated empirically by the skilled test baker ormeasured using a texture analyzer (e.g., TAXT2), as is known in the art.

Examples of chemical anti-staling agents include polar lipids, e.g.,fatty acids and their monoglyceride esters, such as, described in U.S.Pat. No. 4,160,848. In a preferred embodiment, the anti-staling agent isan anti-staling enzyme, which is preferably added to the dough prior tocooking (e.g., baking). Examples of anti-staling enzymes include,without limitation, endo-alpha-amylases, exo-alpha-amylases, such as,e.g., the exo-amylase described in U.S. Pat. No. 6,667,065 and US2004/0043109, pullulanases, glycosyltransferases, amyloglycosidases,branching enzymes (1,4-alpha-glyucan branching enzyme),4-alpha-glucanotransferases (dextrin transferase), beta-amylases,maltogenic alpha-amylases, lipases, phospholipases, galactolipases,acyltransferases, pectate lyases, xylanases, xyloglucanendotransglycosylases, proteases, e.g., as described in WO 2003/084331,peptidases and combinations thereof.

In a preferred embodiment, the anti-staling enzyme is a beta-amylase (EC3.2.1.2). The beta-amylase may be obtained from any suitable sources,such as, plant (e.g. soy bean) or from microbial sources (e.g.Bacillus).

More preferably, the anti-staling enzyme is a maltogenic alpha-amylase(EC 3.2.1.133). The maltogenic alpha-amylases is added into the dough inan amount effective to retard the staling of the product, such as, atleast 500 MANU/flour, more preferably in an amount of at least 500 to1500 MANU/flour. A maltogenic alpha-amylase may be obtained from anysuitable source, such as, derived from a bacteria, such as, Bacillus,preferably, B. stearothermophilus, e.g. from strain NCIB 11837 or avariant thereof made by amino acid modification (EP 494233 B1, U.S. PatNo. 6,162,628). The maltogenic alpha-amylase may preferably be added ata dosage of at least 500 MANU/kg flour, more preferably, at least 750MANU/kg flour, at least 1000 MANU/kg flour (MANU unit defined in U.S.Pat. No. 6,162,628), which is hereby incorporated by reference. Apreferred maltogenic alpha-amylase is NOVAMYL® (available form NovozymesA/S).

In another preferred embodiment, the anti-staling enzyme is an xylanase.The xylanase may be obtained from any suitable source, e.g. fromBacillus, e.g., Bacillus subtilis, as described in WO 2003/010923, WO2001/066711 or WO 2000/039289, and Aspergillus, Trichoderma andThermomyces as described in WO 96/32472.

Optionally, an additional enzyme may be used together with the aboveanti-staling enzymes, such as, e.g., a transglutaminase, a cellulyticenzyme, e.g., a cellulase, an acyltransferase, a protein disulfideisomerase, a pectinase, a pectate lyase, an oxidoreductase, e.g., aperoxidase, a laccase, a glucose oxidase, a pyranose oxidase, a hexoseoxidase, a lipoxygenase, an L-amino acid oxidase or a carbohydrateoxidase. The enzyme may be of any origin, including mammalian, plant,and preferably microbial (bacterial, yeast or fungal) origin and may beobtained by techniques conventionally used in the art.

In preferred embodiments, the microbial inhibition methods of thepresent invention are used in combination with anti-staling agents,preferably, anti-staling enzymes, such as a maltogenic alpha-amylase, toobtain a significant extension in shelf life. In a preferred embodiment,bread having an extended shelf life is prepared by applying apreservative to the surface of a dough comprising an anti-stalingenzyme. In a more preferred embodiment, a bread having an extended shelflife is prepared by applying a preservative in an amount of at least 0.1milligrams active ingredient per cm², more preferably, in the range of0.01 to 5 milligrams/cm², to the outer surface of a dough comprisingmaltogenic alpha-amylase in an effective amount, e.g. more 100 MANU/kgflour, more than 500 MANU/kg flour, such as, between 100 to 1500 MANU/kgflour.

Although the present invention is applicable to producing dough productshaving any desired shelf life, e.g., a shelf life of 7 to 19 days, in apreferred embodiment, the prevent invention is also directed tocommercially acceptable dough based products that are characterized byhaving a longer shelf life then dough based products prepared by othermethods, e.g., a shelf life of above 20 days, above, 21 days, above 22days, above 23 days, above 24 days, above 25 days, above 26 days, above27 days, above 28 days, above 29 days, above 30 days, above 40 days,above 50 days, above 60 days, above 70 days, above 80 days, or above 90days.

In a preferred embodiment, the present invention relates to a method fordistributing baked products, comprising a) producing at a productionfacility a baked product having a shelf life of at least about 22 days;b) transporting the baked product from the production facility to apoint of sale location; c) displaying the baked product for sale at thepoint of sale location, preferably, a plurality of point of salelocations; and wherein the time for achieving a)-c) is for a period ofat least the shelf life of the product. In preferred embodiments, theshelf life of the product is at least about 23 days, at least about 24days, at least about 25 days, at least about 26 days, at least about 27days, at least about 28 days, at least about 29 days, at least about 30days, at least about 40 days, at least about 50 days, at least about 60days, at least about 70 days, at least about 80 days, and at least about90 days. In other preferred embodiments, the time for achieving a)-c) isfor a period of at least 1 day, 2 days or 3 days prior to the expirationof the shelf life of the product. In other preferred embodiments, thetime for achieving b) is up to at least 11 days, 10 days, 9 days, 8days, 7 days, 6 days, 5 days, 4 days, 3 days, 2 days or 1 day prior tothe expiration of the shelf life of the product.

In some preferred embodiments, the baked product is displayed for saleat the point of sale location for a period of 1 day, 2 days, 3 days, 4days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days,13 days, 14 days, 15, days, 16 days, 17 days before the expiration ofthe shelf life. In other preferred embodiments, the baked product isdistributed to the point of sale location at least 1 day, 2 days, 3,days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days,12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18, days, 19 days,20 days, 21 days, 22 days prior to the expiration of the shelf life ofthe product, alternatively, at day 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13,14, 15, 16, 17, 18, 19, 20, 21 or 22 following production. In otherpreferred embodiments, the baked product is displayed at the point ofsale location at day 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,17, 18, 19, 20, 21, or 22 following production.

The baked products may be transported from the production facility tothe point of sale location through at least one distributor, such as, bytrucking the product.

Another preferred embodiment relates to a method for distributing bakedproducts, comprising a) producing at a production facility a bakedproduct having a shelf life of at least about 22 days; b) transportingthe baked product from the production facility to a plurality of pointof sale locations; wherein the plurality of point of sale locationscomprises at least one point of sale location in which the baked goodsare delivered on or after day 18 following production; c) displaying thebaked product for sale at the plurality of point of sale locations for aperiod up to the shelf life of the baked product. In preferredembodiments, the baked product has a shelf life of at least is at leastabout 23 days, at least about 24 days, at least about 25 days, at leastabout 26 days, at least about 27 days, at least about 28 days, at leastabout 29 days, at least about 30 days, at least about 40 days, at leastabout 50 days, at least about 60 days, at least about 70 days, at leastabout 80 days, and at least about 90 days.

Another preferred embodiment relates to a method for distributing bakedproducts comprising a) producing at a production facility a bakedproduct having a shelf life of about 23 days; b) transporting the bakedproduct from the production facility to a plurality of point of salelocations; wherein the transporting may occur during day 2-23 followingproduction; c) displaying the baked product for sale at the plurality ofpoint of sale locations for a period up to the shelf life. Preferably,the baked product has a shelf life of at least is at least about 24days, at least about 25 days, at least about 26 days, at least about 27days, at least about 28 days, at least about 29 days, at least about 30days, at least about 40 days, at least about 50 days, at least about 60days, at least about 70 days, at least about 80 days, and at least about90 days.

Another preferred embodiment relates to a method for distributing bakedproducts, comprising a) producing at a production facility a bakedproduct having a shelf life of at least about 30 days; b) transportingthe baked good from the production facility to a plurality of point ofsale locations; and c) displaying the baked good at the point of salelocation for sale; wherein the transportation and display of the bakedproduct is for a period of up to the shelf life of the product.

Yet another preferred embodiment relates to a method for distributingbaked products, comprising: a) receiving orders for a baked product froma plurality of purchasers; wherein for at least one purchaser of thebaked product, the baked product will not be delivered until at leastabout day 18 following production of the baked product; b) producing ata central production facility a baked product batch to supply theplurality of purchasers, wherein the baked product has a shelf life ofat least about 23 days; c) completing the orders by delivering therequested quantity of backed product to the plurality of purchasers. Inpreferred embodiments, the baked product has a shelf life of is at leastabout 23 days, at least about 24 days, at least about 25 days, at leastabout 26 days, at least about 27 days, at least about 28 days, at leastabout 29 days, at least about 30 days, at least about 40 days, at leastabout 50 days, at least about 60 days, at least about 70 days, at leastabout 80 days, and at least about 90 days.

A further preferred embodiment relates to. a method for distributingbaked products, comprising: a) producing at a production facility abaked product having a shelf life of at least about 22 days; b)distributing the baked product to a plurality of point of sale locationsfor display of the baked product for purchase and consumption; and c)displaying the baked products for purchase; wherein the period availablefor displaying the baked products for purchase begins at least five daysprior to expiration of the shelf life of the baked product. In preferredembodiments, the baked product has a shelf life of at least about 23days, about 24 days, about 25 days, about 26 days, about 27 days, about28 days, about 29 days or about 30 days. In other preferred embodiments,the period available for displaying the baked products for purchasebegins at least 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12days, 13 days, or 14 days prior to expiration of the shelf life of thebaked product.

EXAMPLES Example 1 Pan Release Emulsion

Two pan-release compositions were formulated as follows: Water 50.0%47.0% Sunflower oil 39.2% 36.5% Ca propionate  7.1% 13.0% Emulsifier 3.7%  3.5%  100%  100%

The emulsifier is thermally oxidized and polymerized soyaoil emulsifier(Product name, Palsgaard 4104, available form Palsagaard Industry A/S,Juelsminde, Denmark).

1 g to 2 g of the release agent is applied per pan. The bread baked inthat pan has a weight of approximately 450 g.

Example 2 White Bread

Bread was baked according to the following sponge & dough method.

Recipe % on flour basis Sponge Soya oil 2.5 SSL 0.38 Yeast 5 Wheat flour60 Calcium propionate 0.0 or 0.1% relative to the amount of flour in thesponge Water 62 Dough Ascorbic acid optimized for each flour ADA 20 ppmSalt 2 Glucose 7 (dry substance) Water optimized for each flour Wheatflour 40 Calcium propionate 0.0, 0.15, 0.275 or 0.40% relative to theamount of flour in the dough Novamyl ®, product of 900 MANU NovozymesA/S 10.000 BG: hydrated distilled double 0.5% strength monoglycerides

Sponge

Scaling of ingredients, addition of yeast, water, flour, SSL and oilinto mixer bowl. Mixing 90 rpm for 1 minutes, 150 rpm for 4 minutes. Thesponge is weighed, the temperature is measured and the sponge is placedin a bowl˜fermentation 3 hours at 27° C., 86% RH.

Dough

Addition of ingredients and the sponge into the mixer bowl. The spongeand ingredients are mixed together 90 rpm for 9 minutes. The temperatureis measured, and the dough is scaled into smaller pieces of 430 g each.The dough rests on the table for 10 minutes. Doughs are sheeted, moldedand placed lidded pans with and without addition of calcium propionateto pan release oil.

Preparation of Calcium Propionate Containing Pan Release Oil:

10 g of calcium propionate was suspended on approx 10 ml water and 100ml Acartis alube F-6 pan release oil was added. Approx 1.5 g of the oilwas added to the pan.

Fermentation for 55 minutes at 42° C. and 86% RH. Bread is baked at 224°C. for 15 minutes.

Result

It was observed that higher calcium propionate levels in the doughtended to yield loaves with poorer crumb structure. A subjective flavorevaluation showed that the flavor seemed to worsen with increasedcalcium propionate levels in the dough piece and was independent of theaddition in the pan oil.

The loaves were stored for 30 days after baking. No mold growth wasobserved on any loaves until day 12 after baking. Mold growth was foundto occur later in loaves treated with calcium propionate in the pan oilthan in controls made without calcium propionate in the pan oil but withthe same calcium propionate dosage in the dough and sponge.

Example 3 Pan Oil

A mixture was made of 90 parts by weight of sunflower oil and 10 partsof glycerol tri propionate. It was found that the two liquids were fullymiscible and the mixture was clear.

A baking pan was treated with the mixture. Dough was filled in and bakedto make bread. After storage for 12 days, the result showed that thebread baked in the treated tin was not molding whereas bread baked inthe normal tin showed mold.

Example 4 Measurement of Propionic Acid in the Crust

Bread was baked in lidded pans according to the following sponge & doughmethod.

Recipe % on flour basis Sponge Soya oil 2.5 SSL 0.38 Yeast 5 Wheat flour60 Water 62 Dough Ascorbic acid optimized for each flour ADA 20 ppm Salt2 Glucose 7 (dry substance) Water optimized for each flour Wheat flour40 Calcium propionate 0.25 Novamyl ®, product of 900 MANU Novozymes A/S10.000 BG:

Sponge

Scaling of ingredients, addition of yeast, water, flour, SSL and oilinto mixer bowl. Mixing 90 rpm for 1 minutes, 150 rpm for 4 minutes. Thesponge is weighed, the temperature is measured and the sponge is placedin a bowl˜fermentation 3 hours at 27° C., 86% RH.

Dough

Addition of ingredients and the sponge into the mixer bowl. The spongeand ingredients are mixed together 90 rpm for 9 minutes. The temperatureis measured, and the dough is scaled into smaller pieces of 435 g each.The dough rests on the table for 10 minutes. Doughs are sheeted, moldedand placed lidded pans with and without addition of calcium propionateto the pan release oil.

Preparation of Calcium Propionate Containing Pan Release Oil:

15 g of calcium propionate was suspended on approx 15 ml water and 50 mlAcartis alube F-6 pan release oil was added. The resulting pan releaseoil contains 18.7% (w/w) calcium propionate. Approx 1.5 g of the oil wasadded to the pan.

Fermentation for 55 minutes at 42° C. and 86% RH. Bread was baked at200° C. for 22 minutes.

Propionic Acid and Propionate was Measured in the Crust by the FollowingMethod:

The outer layer of the crust (0.5-1 mm) was removed by a kitchen grater.A total of 4 g crust was extracted from each bread followed by thepropionic acid measurement following the method for extraction anddetermination of propionic acid described by M J Scotter et al (FoodAdditives and contaminants (1994) 11: 295-300). The amount of propionicacid was measured relative to a propionic standard, and the results areexpressed as gram calcium propionate per kg of bread.

The results were as follows:

Bread with Calcium Propionate in the Pan Release Mix:

-   CaPro in the crust: 2.28 g/kg (mean of 3 measurements)-   CaPro in the crumb: 1.00 g/kg (mean of 2 measurements)    Bread without Calcium Propionate in the Pan Release Mix:-   CaPro in the crust: 0.06 g/kg (mean of three measurements)

It is seen that only a very low amount of propionic acid is measured inthe crust from bread baked without calcium propionate in the pan releaseoil—this is likely because of extensive evaporation of propionic acidfrom the crust.

A higher amount of calcium propionate is measured in the crust of thebread when calcium propionate is added into the pan release oil, alsocompared to the amount in the crumb.

Example 5 Water-In-Oil Emulsion Mold-Inhibitor Composition

A thermally oxidized and polymerized soyaoil emulsifier (Product name,Palsgaard 4104, available from Palsgaard Industry A/S, Juelsminde,Denmark) is placed in a beaker and stirred.

Add warm water (50-60° C.) and the desired amount of ananti-preservative (e.g., calcium propionate). The solution is pouredslowly at first and then quickly to produce an oil/emulsifier blendwhile stirring at maximum speed. Stir for two minutes after the wholewater phase is added.

Homogenize the composition for 2-3 minutes, although the homogenizationtime depends on the ratio between the size of the stirrer and thedimension of the beaker.

Example 6 Finely Dispersed Preservative Agents

The concentration of the preservative calcium propionate in the crust ofbread after treatment with a composition comprising finely dispersedcalcium propionate preservative particles according to the presentinvention was evaluated.

Breads were baked in open pans according to the sponge and doughstandard procedure and with addition of 0.10% (relative to the amount offlour) Ca-propionate in the sponge and 0.10% Ca-propionate in the dough.900 MANU Novamyl/kg was also added.

Dough Pan oils 1 2 3 4 5 Superfine 0 15% 15% 15% Ca-propionate Triacetin(TA) 0 0 10% 20% K-sorbate 15%

The pan oils are based on (Trennaktiv PR 100 pan oil from Dubor).Ca-propionate was a superfine quality (FCC, food chemical codex) andpotassium sorbate was a standard quality. Sufficient pan oil was addedto ensure a uniform layer—the actual amount of pan oil was recorded (bythe delta weight of the pans). The outer layer of the crust (0.5-1 mm)was removed by a grater. A total of 5 g crust was extracted from eachbread followed by the propionic acid measurement using the method forextraction and determination of propionic acid described by M J Scotteret al (Food Additives and contaminants (1994) 11: 295-300). The amountof propionic acid was measured relative to a propionic acid standard,and the results are expressed as a calcium propionate per kg of bread.

In dough 5, the amount of sorbic acid was measured following the sameextraction and analysis procedure, the results are expressed in gram ofpotassium sorbate per kg bread.

Propionic acid was measured one day after baking from the sides andbottom of the crust (from 3 breads) and from the crumb (from 2 breads).Furthermore, propionic acid was measured from the top of the crust ofbread 3. pH was measured after suspension in 10 times water (w/w) on thesame crust/crumb samples as were used for extraction of propionic acid.

The results (mean values) of the propionic acid and sorbic acidmeasurements (in g calcium propionate/kg bread or g potassium sorbate/kgbread) are given in the table below.

Dough 3 Dough 4 Dough 4 Dough 1 Dough 2 15% CaP 15% CaP 15% reference15% CaP 10% TA 15% TA K-Sorbate Ca-P in crust (sides and 0.09 0.91 1.101.10 — bottom) Ca-P in crust (top) — — 0.12 — — Ca-P in crumb 0.82 0.760.76 0.88 — k-sorbate in crust (sides 0 — — — 0.32 and bottom) K-sorbatein the crumb 0 — — — 0.05

It is observed that the pan oil with calcium propionate or potassiumsorbate significantly increased the amount of preservative in the crustwhich has been in contact with the pan.

Example 7 Surface Treatment with a pH Adjusting Agent

An experiment was performed to determine the effect of the combinationof a pH adjusting agent (triacetin) and a preservative (calciumpropionate) to the surface of a dough based product. Bread was bakedaccording to the following sponge & dough method:

Mixing: Sponge; 3 min. Slow and 2 min fast in the Spiral mixer 24° C.,Sponge time 3 hours at room temperature Dough; 5.5 min. high speed inthe Morton Z-blade mixer. Scale one dough piece and put the rest of thedough through the meat mill.

Ingredients Sponge % Dough % USA flour 58 42 Water 35 22 Fermipan Brown2 1 Ascorbic acid 0.0025 — Salt — 2 HFCS (73% solids) — 15 Oil — 3 SSL0.5 — Emulsifier 0.5 — Calcium propionate 0.1 — Rotox — 0.05 Softase —0.5

-   Dough temperature: 27° C.-   Dough weight: 400 g. Round up softly by hand.-   Proofing time: 6 min. at room temp.-   Moulding: With the Mono Emulsifier Walls; 4 Pressure belt; 7    Conductors; 9;-   Final Proofing time: 70 min. at 40° C. and 80% R.H.;-   Baking: 21 min. at Top 200° Floor 250° C.

Various concentrations of a preservative (calcium propionate), with andwithout the pH adjusting agent (triacetin), were included as componentsin a pan oil release composition which was sprayed onto a baking pan,using a Preval sprayer (2.1 oz contents under pressure) with a 150 mlbottle for the release agent. 0.1% of the preservative calciumpropionate was also added into the sponge of the dough. The calciumpropionate was sieved with the release agent before use with a 200micron sieve. pH measurements were taken with 7 day old bread, stored inclosed plastic bags following baking, by slicing 1.5 to 2 millimetresfrom the bottom and sides of the crust, with a total of 25 grams (orapproximately 5% of the total bread weight). The pH measurements wereperformed by milling the 25 grams of the bread crust. 15 grams of thebread crust was mixed with 135 grams of neutral water and the pH wasmeasured using a pH meter (PHX 1495). As shown below, the addition ofthe pH adjusting agent compensated for the increase in pH in the surfaceof the dough product caused by the preservative.

Trial 1 Trail 2 Trial 3 Trial 4 Trial 5 Trial 6 (0% calcium (10% (15%(15% (15% (10% propionate calcium calcium calcium calcium calcium and 0%propionate propionate propionate propionate propionate triacetin) and 0%and 0% and 10% and 20% and 30% triacetin) triacetin) triacetin)triacetin) triacetin) pH: 5.49 pH: 5.7 pH: 5.83 pH: 5.75 pH: 5.66 pH:5.42

Example 8

In this experiment, the level of high fructose corn syrup normally usedin bread commonly prepared for the U.S. (15% to 20%) market was reducedsignificantly (5%) in order to further observe the anti-moldeffectiveness of the present invention. In particular, the breadcontained 0.1% of calcium propionate in the dough with 5% high fructosecorn syrup.

The bread was prepared by the following recipe

Ingredients Sponge grams Dough grams USA flour 910 390 Water 507 260Ascorbic Acid 0.034 — Compressed yeast 26 — Ammonium sulphate 0.6 — Oil52 — SSL 1.62 — Fermipan Brown (dried 19.5 6.5 yeast, DSM, Neatherlands)HFCS — 91 Rotox (oxidant) (ICS) — 0.81 Softase (softner) (ICS) — 6.5Propionate 1.4 grams in sponge (=0.1% on flour weight).

Processing:

-   Mixing: Sponge; 3 min. Slow and 2 min. Fast in the Spiral mixer 24°    C.    -   Sponge time, 2.5-3 hours at room temperature    -   Dough: ½ min., slow speed in Mc. Duffy mixer, 8½ min. high speed        in Mc. Duffy mixer-   Dough temp: 26° C.-   Rest time: 2 min. at room temp.-   Dough wt. 420 g-   To round up softly by hand.-   Proofing time: 6 min. at room temp.-   Molding: With the “Mono” walls, 3.5 pressue belt, 6.5 conductors, 9-   Final Proofing time: variable (normally 75 min. at 40° C.)    -   And 80% R.H.    -   Baking: 20 min. @210° C. (top), 230° C. bottom

The dough for the bread samples were then treated prior to baking withthe following compositions:

1: Control: no surface treatment with a preservative (calcium propionate(CP) or potassium sorbate (PS)) or a pH adjusting agent (triacetin (TA))

2: 10% CP +30% TA

3: 10% PS

4: 10% PS+15% TA

5: 10% PS+30% TA

6: 3% PS

7: 3% PS+15% TA

The bread samples were then evaluated at days 8, 9, 10, 11, 12, 13, 14and 15 post-baking under the same storage conditions for mold formationand rated as follows:

Rating Mold Level 10 No Mold 9 there might be 1 spot in 4 loaves, butdifficult to see 8 1 spot on 1 loaf out of 4 7 2 spots on 1 loaf or 1spot on 2 loaves 6 3 spots total 5 4 spots in max 3 loaves 4 4 spots on4 loaves or 5 to 7 spots in 1 to 4 loaves 3 8 to 11 spots 2 11 to 15spots 1 more than 15 spots

The results were as follows:

Day 8 9 10 11 12 13 14 15 Control 10 9 7 6 5 3 1 1 10% CP + 30% TA 10 1010 10 10 9 8 7 10% PS 10 10 10 10 9 8 6 6 10% PS + 15% TA 10 10 10 10 109 8 7 10% PS + 30% TA 10 10 10 10 10 10 9 8  3% PS 10 10 8 7 6 5 3 1  3%PS + 15% TA 10 10 10 10 8 8 7 6

As is clearly shown above, the surface treatment of the dough prior tobaking with a preservative significantly improved the mold resistance ofthe bread when compared to the control, however, the combination of thepreservative and the pH adjusting agent (triacetin) surface treatmentwas significantly more effective than the surface treatment with apreservative alone.

Example 9

Breads were prepared and evaluated at days 8, 9, 10, 11, 12, 13, 14 and15 post-baking for mold formation as described in Example 8, with thefollowing surface application of the dough prior to baking:

1: Control: no surface treatment with a preservative (calcium propionate(CP) or citric acid (CA) or mono calcium propionate (MCP)) or a pHadjusting agent (triacetin (TA))

2: 10% CP+30% TA

3: 10% CP+10% CA

4: 10% CP+5% CA

5: 10% CP/5% CA/10% TA

6: 15% CP+10% MCP

Day 8 9 10 11 12 13 14 15 Control 10 9 7 6 5 3 1 1 10% CP 30% TA 10 1010 10 10 9 8 7 10% CP 10% CA 10 10 10 10 8 7 6 4 10% CP 5% CA 10 10 9 87 6 5 4 10% CP 5% CA 10 10 10 9 8 7 6 5 10% TA 15% CP 10% MCP 10 10 8 76 6 3 1

As is clearly shown above, the surface treatment of the dough prior tobaking with various combinations of preservatives and/or a pH adjustingagent (triacetin) improved the mold resistance of the bread, however,the surface treatment with the combination of a preservative and a pHadjusting agent triacetin was the most effective.

Example 10

Breads were prepared and evaluated at days 8, 9, 10, 11, 12, 13, 14 and15 post-baking for mold formation as described in Example 8, with thefollowing surface application of the dough prior to baking:

1: Control: no surface treatment with a preservative (calcium propionate(CP)) or a pH adjusting agent (triacetin (TA))

2: 10% CP+30% TA

3: 5% CP+15% TA

4: 15% CP+15% TA

5: 15% CP

Day 8 9 10 11 12 13 14 15 control 10 9 7 6 5 3 1 1 10% CP 30% TA 10 1010 10 10 9 8 7  5% CP 15% TA 10 10 10 9 8 7 6 4 15% CP 15% TA 10 10 1010 9 8 6 5 15% CP 10 10 9 8 7 6 5 3

Again, as is clearly shown above, the surface treatment of the doughprior to baking with a preservative and the pH adjustng agent(triacetin) significantly improved the mold resistance of the bread.

Example 11 Pan Release Composition

A pan release composition was prepared as follows: Dubor Trennaktiv PR100, an oil for all baking trays and tins that contain wax and lecithinwas mixed at room temperature with fine particle size Ca-propionate andtriacetin in different ratios using a lab stirrer to obtain a releasecomposition that was than used to grease the baking pan. The dough isthan deposited in the pan and baked.

Example 12 Pan Release Composition

A pan release composition may be prepared as follows: Blend carnauba waxand lecithin with a liquid oil at a temperature above the melting pointof the wax and after a clear solution is reached the mixture is cooleddown while stirring till room temperature is reached. The mixture canthan be used to prepare preservative compositions, as described herein.

1-78. (canceled)
 79. A process for preparing bread, comprising: (a)treating a surface of a dough which is in contact with a pan used tobake said dough with at least one pH adjusting agent in an amounteffective to inhibit microbial growth on the surface of the breadprepared from the dough wherein the at least one pH adiusting agent isadded in an amount effective to lower the pH of the surface of the doughand/or bread prepared from the dough by a factor of at least 0.01 pHunits; and (b) baking the dough to form the bread; wherein the treatingis done prior to or during baking.
 80. The process of claim 79, whereinthe pH adjusting agent is solid or liquid agent which is converted to agas or vapor during the baking or a heat activated pH adjustingsubstance which is broken down at baking temperatures into one or morecomponents which will cause a reduction in pH of the treated surface.81. The process of claim 79, wherein the at least one pH adjusting agentis selected from the group consisting of triacetin, monocalciumphosphate, acetic acid, citric acid, pyrophosphate, sodium phosphate,potassium phosphate, and combinations thereof.
 82. The process of claim79, wherein the at least one pH adjusting agent is triacetin.
 83. Theprocess of claim 79, further comprising treating the surface of thedough with at least one preservative in an amount effective to inhibitmold growth on the surface of the bread prepared from the dough.
 84. Theprocess of claim 83, wherein the at least one preservative is selectedfrom the group consisting of sodium benzoate, calcium benzoate,potassium benzoate, sodium diacetate, paraben, niacin, calcium acetate,calcium diacetate, sodium sorbate, calcium sorbate, potassium sorbate,sodium propionate, calcium propionate, potassium propionate, sorbicacid, acetic acid and combinations thereof.
 85. The process of claim 79,wherein the at least one pH adjusting agent is in the form of an aqueouscomposition.
 86. The process of claim 79, wherein the at least one pHadjusting agent is in the form of an oil-based composition.
 87. Theprocess of claim 86, wherein the oil based composition is a water-in-oilemulsion.
 88. The process of claim 86, wherein the oil based compositionis an oil-in-water emulsion.
 89. The process of claim 86, wherein theoil based composition is a mixture of oils, waxes and lecithins.
 90. Theprocess of claim 86, wherein the at least one pH adjusting agent issuspended in the composition in the form of very fine particles having amean particle size below 200 μm.
 91. The process of claim 86, whereinthe at least one pH adjusting agent is suspended in the composition inthe form of very fine particles having a mean particle size below in therange between 10 μm and 200 μm.
 92. The process of claim 86, wherein thecomposition further comprises fatty particles or wax particles.
 93. Theprocess of claim 79, wherein the wherein the at least one pH adjustingagent is a component of a pan release composition comprising at leastone pan release agent, and the treating comprises treating the surfaceof pan used in baking the dough with said pan release composition. 94.The process of claim 79, wherein the process further comprises treatingthe dough with at least one flavoring agent or at least one flavorprecursor.
 95. The process of claim 94, wherein the at least oneflavoring agent is selected from the group consisting of a butterflavor, bread flavor, bread crust flavor, bread crumb flavor, vanillaflavor, and meat flavor.
 96. The process of claim 79, wherein the atleast one pH adiusting agent is a component of a treating compositionfurther comprising a pH adjusting agent and at least one flavoring agentor at least one flavor precursor.
 97. The process of claim 79, whereinthe at least one pH adjusting agent is a component of a treatingcomposition further comprising at least one flavoring agent selectedfrom the group consisting of a butter flavor, bread flavor, bread crustflavor, bread crumb flavor, vanilla flavor, and meat flavor. 98-135.(canceled)